Buffer 2 - Calorimetry


Calorimetry analysis of protein-protein interactions

Tris-Cl pH 8.0 - MgCl2

Composition 

Concentration Ingredient
50 mM

Tris-Cl pH 7.4
100 mM

NaCl
5 mM

MgCl2

Note 

Filter and degas.

Calculator 

1000
Amount to add Ingredient
50.0 ml Tris-Cl pH 7.4, 1 M
20.0 ml Sodium Chloride, 5 M
5.0 ml Magnesium Chloride, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 79