Bradford Reagent


Protein concentration assay

Coomassie Brilliant Blue G-250 - Ethanol - Phosphoric Acid

Composition 

Concentration Ingredient
0.01 % (wt:vol)

Coomassie Brilliant Blue G-250
4.75 % (vol:vol)

Ethanol
8.55 % (wt:vol)

H3PO4

Note 

Filter through Whatman No. 1 after dissolution.

Calculator 

1000
Amount to add Ingredient
100.0 mg Coomassie Brilliant Blue G-250 (solid)
50.0 ml Ethanol, 95% (vol:vol)
100.0 ml Phosphoric Acid, 85.5% (wt:vol)
Storage
Temperature
  • 4°C
Estimated Shelf Life
  • 3 months


Reference(s)
Reference Title PMID/ISBN Note
Gallagher and Wiley (eds.) 2012 Current Protocols Essential Laboratory Techniques 978-0470942413 p. 2.2.34