BPTE Electrophoresis Buffer, 10X


Hybridization analysis of nucleic acids

PIPES - Bis-Tris - EDTA

Composition 

Concentration Ingredient
100 mM

PIPES
300 mM

Bis-Tris Methane
10 mM

EDTA pH 8.0

Note 

After additon of all ingredients, treat with DEPC and autoclave

Calculator 

100
Amount to add Ingredient
3.02 g PIPES (solid)
6.28 g Bis-Tris Methane (solid)
2000.0 ul EDTA pH 8.0, 0.5 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 14