Bouin's Fixative

Preparation of parrafin tissue sections

Dissolve picric acid in a volume of water equal to half of final volume. Filter through Whatman No. 1 paper or equivalent. Add paraformaldehyde and heat to 60C in a fume hood. Add a few drops of 1 N NaOH to help dissolution. Cool to room temperature and add remaining ingredients.

Composition

Concentration	Ingredient
0.2 % (wt:vol)	Picric Acid
2 % (wt:vol)	Paraformaldehyde
1 X	PBS

Calculator

Amount to add	Ingredient
2.00 g	Picric Acid (solid)
20.00 g	Paraformaldehyde (solid)
100 ml	PBS, 10 X

Reference(s)

Reference	Title	PMID/ISBN	Note
Greenfield (ed.) 2014	Antibodies, A Laboratory Manual, 2nd Edition	9781936113811	p. 699