Bodian's No. 2 Fixative


Sample fixation

Formaldehyde - Acetic Acid - Ethanol

Composition 

Concentration Ingredient
1.85 % (wt:vol)

Formaldehyde
5 % (vol:vol)

Acetic Acid
72 % (vol:vol)

Ethanol

Calculator 

50
Amount to add Ingredient
2.5 ml Formaldehyde, 37% (wt:vol)
2.5 ml Acetic Acid - Glacial, 100% (vol:vol)
36.0 ml Ethanol, 100% (vol:vol)
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 101