Blunt-end Ligation Buffer, 10X


Blunt-end ligation of DNA fragments

Tris-Cl pH 7.6 - MgCl2 - DTT - BSA - Hexamminecobalt Chloride - ATP - Spermidine HCL

Composition 

Concentration Ingredient
660 mM

Tris-Cl pH 7.6
50 mM

MgCl2
50 mM

DTT
1 mg/ml

BSA
100 mM

[Co(NH3)6]Cl3
2 mM

ATP Disodium Salt
5 mM

Spermidine

Calculator 

10
Amount to add Ingredient
6.6 ml Tris-Cl pH 7.6, 1 M
500.0 ul Magnesium Chloride, 1 M
500.0 ul DTT, 1 M
1000.0 ul Bovine Serum Albumin, 10 mg/ml
10.0 ml Hexamminecobalt(III) Chloride, 100 mM
20.0 ml Adenosine Triphosphate Disodium Salt, 10 mM
500.0 ul Spermidine, 100 mM
Storage
Temperature
  • -20°C


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 38