BLOTTO


Blocking solution for Western blotting

Nonfat Dried Milk - Antifoam A - Sodium Azide - PBS

Composition 

Concentration Ingredient
5 % (wt:vol)

Milk, Bovine Non-Fat Dry
0.01 % (vol:vol)

Antifoam A Concentrate
0.02 % (wt:vol)

NaN3
1 X

PBS

Calculator 

100
Amount to add Ingredient
5.00 g Milk, Bovine Non-Fat Dry (solid)
10.0 ul Antifoam A Concentrate, 100% (vol:vol)
200.0 ul Sodium Azide, 10% (wt:vol)
10 ml PBS, 10 X


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 83