Blocking Solution - Phage Display


Phage display

PBS - Milk - PMSF - Benzamidine

Composition 

Concentration Ingredient
150 mM

NaCl
20 mM

Sodium Phosphate Buffer pH 7.4
5 % (wt:vol)

Milk, Bovine Non-Fat Dry
0.5 mM

PMSF
0.2 mM

Benzamidine Hydrochloride

Calculator 

1000
Amount to add Ingredient Special Instructions
30.0 ml Sodium Chloride, 5 M
20.0 ml Sodium Phosphate Buffer pH 7.4, 1 M
50.00 g Milk, Bovine Non-Fat Dry (solid)
5.0 ml PMSF, 100 mM Add just before use
200.0 ul Benzamidine Hydrochloride, 1 M Add just before use


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 76