Biocytin 0.5% (wt:vol) in Pipette Solution | Nectagen

Biocytin 0.5% (wt:vol) in Pipette Solution

Ballistic delivery of dyes to neurons

Biocytin - Pipette Solution

Composition

Concentration	Ingredient
120 mM	Potassium Gluconate
10 mM	KCl
1 mM	MgCl₂
10 mM	HEPES
0.1 mM	EGTA pH 7.0
25 uM	CaCl₂
1 mM	ATP
0.2 mM	GTP
4 mM	Glucose
0.5 % (wt:vol)	Biocytin

Calculator

Amount to add	Ingredient
60.0 ul	Potassium Gluconate, 2 M
10.0 ul	Potassium Chloride, 1 M
1.0 ul	Magnesium Chloride, 1 M
10.0 ul	HEPES, 1 M
0.1 ul	EGTA pH 7.0, 1 M
0.0 ul	Calcium Chloride, 1 M
10.0 ul	ATP, 100 mM
2.0 ul	GTP, 100 mM
2.0 ul	Glucose, 2 M
5.0 mg	Biocytin (solid)

Storage
Estimated Shelf Life	None (Use fresh)

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 50