Binding Buffer - Phage Display | Nectagen

Binding Buffer - Phage Display

Phage display

Tris-Cl pH 7.5 - KCl - MgCl₂ - DTT - Glycerol - Milk - Tween-20 . Pefabloc SC - Benzamidine - Aprotinin - Leupeptin - NaF - Na₃VO₄

Composition

Concentration	Ingredient	Details
25 mM	Tris-Cl pH 7.5
100 mM	KCl
5 mM	MgCl₂
1 mM	DTT
5 % (vol:vol)	Glycerol
1 % (wt:vol)	Milk, Bovine Non-Fat Dry
0.1 % (vol:vol)	TWEEN® 20
0.4 mM	Pefabloc SC	Also known as AEBSF
0.1 mM	Benzamidine Hydrochloride
1 ug/ml	Aprotinin
1 ug/ml	Leupeptin
1 mM	NaF
0.1 mM	Na₃VO₄

Calculator

Amount to add	Ingredient
25.0 ml	Tris-Cl pH 7.5, 1 M
100.0 ml	Potassium Chloride, 1 M
5.0 ml	Magnesium Chloride, 1 M
1000.0 ul	DTT, 1 M
50.0 ml	Glycerol, 100% (vol:vol)
10.00 g	Milk, Bovine Non-Fat Dry (solid)
10.0 ml	TWEEN® 20, 10% (vol:vol)
2000.0 ul	Pefabloc SC, 200 mM
100.0 ul	Benzamidine Hydrochloride, 1 M
100.0 ul	Aprotinin, 10 mg/ml
1000.0 ul	Leupeptin, 1 mg/ml
1000.0 ul	Sodium Fluoride, 1 M
500.0 ul	Sodium Orthovanadate, 200 mM

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 77