Binding Buffer - IMAC


Immobilized metal-ion affinity chromatography

Sodium Phosphate pH 7.4 - NaCl - Low Imididazole

Composition 

Concentration Ingredient
20 mM

Sodium Phosphate Buffer pH 7.4
0.5 M

NaCl
20 mM

Imidazole

Calculator 

500
Amount to add Ingredient
10.0 ml Sodium Phosphate Buffer pH 7.4, 1 M
50.0 ml Sodium Chloride, 5 M
10.0 ml Imidazole, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 59