BCA Reagent A


Component of BCA protein concentration assay

Bicinchoninic Acid - Na2CO3 - Sodium Tartrate - NaOH - NaHCO3

Composition 

Concentration Ingredient
1 % (wt:vol)

BCA
2 % (wt:vol)

Na2CO3
0.16 % (wt:vol)

Sodium Tartrate
0.4 % (wt:vol)

NaOH
0.95 % (wt:vol)

NaHCO3

Note 

Adjust to pH 11.25 with 50% NaOH

Calculator 

10
Amount to add Ingredient
100.0 mg Bicinchoninic Acid Disodium Salt Hydrate (solid)
200.0 mg Sodium Carbonate Monohydrate (solid)
16.0 mg Sodium Tartrate Dihydrate (solid)
40.0 mg Sodium Hydroxide (solid)
95.0 mg Sodium Bicarbonate (solid)
Storage
Temperature
  • Room Temperature
Estimated Shelf Life
  • Indefinite


Reference(s)
Reference Title PMID/ISBN Note
Gallagher and Wiley (eds.) 2012 Current Protocols Essential Laboratory Techniques 978-0470942413 p. 2.2.34