Basic Buffer


Use and labeling of GST fusions

HEPES pH 7.15 - KCl - MgCl2 - DTT - IGEPAL CA-630

Composition 

Concentration Ingredient
20 mM

HEPES pH 7.5
50 mM

KCl
10 mM

MgCl2
1 mM

DTT
0.1 % (vol:vol)

IGEPAL® CA-630

Calculator 

10
Amount to add Ingredient
200.0 ul HEPES pH 7.5, 1 M
500.0 ul Potassium Chloride, 1 M
100.0 ul Magnesium Chloride, 1 M
10.0 ul DTT, 1 M
100.0 ul IGEPAL CA-630, 10% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 75