Assembly Buffer


Imaging intermediate filament proteins in living cells

Sodium/Potassium Phosphate pH 7.4 - NaCl - KCl - β-mercaptoethanol - PMSF

Composition 

Concentration Ingredient
6 mM

Sodium Phosphate Buffer pH 7.4
0.17 M

NaCl
3 mM

KCl
0.2 % (vol:vol)

β-mercaptoethanol
0.2 mM

PMSF

Calculator 

1000
Amount to add Ingredient
6.0 ml Sodium Phosphate Buffer pH 7.4, 1 M
34.0 ml Sodium Chloride, 5 M
3.0 ml Potassium Chloride, 1 M
2000.0 ul β-Mercaptoethanol, 100% (vol:vol)
2000.0 ul PMSF, 100 mM
Storage
Estimated Shelf Life
  • None (Use fresh)


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 46