Artificial Cerebrospinal Fluid


Artitifical Cerebrospinal Fluid

NaCl - KCl - MgSO4 - KH2PO4 - NaHCO3 - Glucose - Sucrose - CaCl2

Composition 

Concentration Ingredient
124 mM

NaCl
2.5 mM

KCl
2 mM

MgSO4
1.25 mM

KH2PO4
26 mM

NaHCO3
10 mM

Glucose
4 mM

Sucrose
2.5 mM

CaCl2

Note 

Solution may be milky but will clear after equilibration with 95% O2-5% CO2

Calculator 

1000
Amount to add Ingredient
24.8 ml Sodium Chloride, 5 M
2.5 ml Potassium Chloride, 1 M
2000.0 ul Magnesium Sulfate, 1 M
1250.0 ul Potassium Phosphate Monobasic, 1 M
26.0 ml Sodium Bicarbonate, 1 M
5.0 ml Glucose, 2 M
2000.0 ul Sucrose, 2 M
2.5 ml Calcium Chloride, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 45