AP 9.5


Detection of probes labeled with alkaline phosphatase

Tris-Cl pH 9.5 - NaCl - MgCl2

Composition 

Concentration Ingredient
0.1 M

Tris-Cl pH 7.5
0.1 M

NaCl
50 mM

MgCl2

Calculator 

1000
Amount to add Ingredient
100.0 ml Tris-Cl pH 7.5, 1 M
20.0 ml Sodium Chloride, 5 M
50.0 ml Magnesium Chloride, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Spector and Goldman (eds.) 2006 Basic Methods in Microscopy 0879697512 p. 230