Antigen Electrophoresis Buffer


Electroelution of protein antigens from polyacrylamide gel slices

Tris-Acetate pH 7.4 - SDS - Sodium Thioglycolate

Composition 

Concentration Ingredient
50 mM

Tris-Acetate pH 7.4
0.1 % (wt:vol)

SDS
0.5 mM

Sodium Thioglycolate

Calculator 

1000
Amount to add Ingredient
50.0 ml Tris-Acetate pH 7.4, 1 M
10.0 ml SDS, 10% (wt:vol)
57.1 mg Sodium Thioglycolate (solid)


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 80