Antigen Dialysis Buffer


Electroelution of protein antigens from polyacrylamide gel slices

Tris-Acetate pH 7.4 - SDS - DTT

Composition 

Concentration Ingredient
2 M

Tris Base
1 % (wt:vol)

SDS
100 mM

DTT

Note 

Adjust pH to 7.4 with acetic acid

Calculator 

1000
Amount to add Ingredient
242.28 g Tris Base (solid)
100.0 ml SDS, 10% (wt:vol)
100.0 ml DTT, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 80