Annealing Buffer, 2X


RNAi in mammalians

Potassium Acetate - Magnesium Acetate - HEPES-KOH pH 7.4

Composition 

Concentration Ingredient
60 mM

HEPES-KOH pH 7.4
200 mM

Potassium Acetate
4 mM

PBS

Calculator 

100
Amount to add Ingredient
6.0 ul HEPES-KOH pH 7.4, 1 M
2.5 ul Potassium Acetate, 8 M
ul PBS, 10 X


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 31