Annealing Buffer, 10X


Nuclease S1 RNA protection

Tris-Cl pH 7.5 - MgCl2 - NaCl - DTT

Composition 

Concentration Ingredient
100 mM

Tris-Cl pH 7.5
100 mM

MgCl2
500 mM

NaCl
100 mM

DTT

Calculator 

250
Amount to add Ingredient
25.0 ml Tris-Cl pH 7.5, 1 M
25.0 ml Magnesium Chloride, 1 M
25.0 ml Sodium Chloride, 5 M
25.0 ml DTT, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 24