AMV-RT, 10X


Isolation and amplification of RNA from single cells and small amounts of tissue

Tris-Cl pH 8.3 - KCl - MgCl2

Composition 

Concentration Ingredient
500 mM

Tris-Cl pH 8.3
1.2 M

KCl
100 mM

MgCl2

Calculator 

1000
Amount to add Ingredient
500.0 ml Tris-Cl pH 8.3, 1 M
89.46 g Potassium Chloride (solid)
100.0 ml Magnesium Chloride, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 13