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Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis
Ampholyte (pH 6.0-8.0 or other narrow range) - Ampholyte (pH 3.5-10.0 or other wide range)
Concentration | Ingredient | Details |
---|---|---|
80 % (vol:vol) |
Pharmalyte pH 5.0-8.0 |
or other narrow pH range |
20 % (vol:vol) |
Pharmalyte pH 3.0-10.0 |
or other wide pH range |
Amount to add | Ingredient |
---|---|
400.0 ul | Pharmalyte pH 5.0-8.0, 100% (vol:vol) |
100.0 ul | Pharmalyte pH 3.0-10.0, 100% (vol:vol) |
Reference | Title | PMID/ISBN | Note |
---|---|---|---|
Greenfield (ed.) 2014 | Antibodies, A Laboratory Manual, 2nd Edition | 9781936113811 | p. 762 |