Ammonium Sulfate Cut Dilution Buffer (ASCDB)

Purification of proteins and inhibitors from cell culture

Tris-Cl pH 7.2 - DTT

Composition

Concentration	Ingredient
20 mM	Tris-Cl pH 7.2
1 mM	DTT

Amount to add	Ingredient
20.0 ml	Tris-Cl pH 7.2, 1 M
1000.0 ul	DTT, 1 M

Storage
Estimated Shelf Life	None (Use fresh)

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 69