Alkaline Running Buffer

RNA electrophoresis running buffer

NaOH - EDTA

Composition

Concentration	Ingredient
50 mM	NaOH
1 mM	EDTA pH 8.0

Use DEPC-treated water

Amount to add	Ingredient
5.0 ml	Sodium Hydroxide, 10 M
2000.0 ul	EDTA pH 8.0, 0.5 M

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 64