Alkaline Phosphatase Staining Solution


Alkaline phosphatase staining of mouse embryos

Tris-Maleate pH 9.0 - α-Naphthyl Phosphate Disodium Salt - Fast Red TR - MgCl2

Composition 

Concentration Ingredient
25 mM

Tris-Maleate pH 9.0
0.4 mg/ml

α-Naphthyl Phosphate Disodium Salt
1 mg/ml

Fast Red TR 1,5-Naphthalenedisulfonate Salt
8 mM

MgCl2

Calculator 

50
Amount to add Ingredient
1250.0 ul Tris-Maleate pH 9.0, 1 M
400.0 ul α-Naphthyl Phosphate Disodium Salt, 50 mg/ml
2.5 ml Fast Red TR 1,5-Naphthalenedisulfonate Salt, 20 mg/ml
400.0 ul Magnesium Chloride, 1 M


Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 109