Alkaline Lysis Plasmid Miniprep Solution II

Minpreparation of plasmid DNA from E. coli

NaOH - SDS

Composition

Concentration	Ingredient
0.2 M	NaOH
1 % (wt:vol)	SDS

Amount to add	Ingredient
20.0 ul	Sodium Hydroxide, 10 M
100.0 ul	SDS, 10% (wt:vol)

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 44