Alkaline Lysis Plasmid Miniprep Solution I


Minpreparation of plasmid DNA from E. coli

Glucose - Tris-Cl pH 8.0 - EDTA

Composition 

Concentration Ingredient
50 mM

Glucose
25 mM

Tris-Cl pH 8.0
10 mM

EDTA pH 8.0

Calculator 

100
Amount to add Ingredient
2.5 ml Glucose, 2 M
2.5 ml Tris-Cl pH 8.0, 1 M
2000.0 ul EDTA pH 8.0, 0.5 M
Storage
Temperature
  • 4°C


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 44