Alkaline Loading Buffer, 6X


Loading dye for electrophoresis

NaOH - EDTA - Ficoll Bromocresol Green - Xylene Cyanol FF

Composition 

Concentration Ingredient
300 mM

NaOH
6 mM

EDTA pH 8.0
18 % (wt:vol)

Ficoll Type 400
0.15 % (wt:vol)

Bromocresol Green
0.25 % (wt:vol)

Xylene Cyanol FF

Calculator 

10
Amount to add Ingredient
300.0 ul Sodium Hydroxide, 10 M
120.0 ul EDTA pH 8.0, 0.5 M
1.80 g Ficoll Type 400 (solid)
15.0 mg Bromocresol Green (solid)
25.0 ml Xylene Cyanol FF, 1 mg/ml


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 68