Agarose Gel, 0.3% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb

Agarose - TBE

Composition

Concentration	Ingredient
0.3 % (wt:vol)	Agarose
1 X	TBE

Efficient range of separation for linear DNA molecules: 5 to 60 kb. Add TBE, 5X after melting agarose completely and cooling slightly.

Amount to add	Ingredient
3.00 g	Agarose - Electrophoresis Grade
200 ml	TBE, 5X

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 65