Agarose Gel, 0.3% (wt:vol) + 0.5X TBE


DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb

Agarose - TBE

Composition 

Concentration Ingredient
0.3 % (wt:vol)

Agarose
0.5 X

TBE

Note 

Efficient range of separation for linear DNA molecules: 5 to 60 kb.  Add TBE, 5X after melting agarose completely and cooling slightly.

Calculator 

100
Amount to add Ingredient
300.0 mg Agarose - Electrophoresis Grade
10 ml TBE, 5X


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 65