Acrylamide Gel, 5.0% (wt:vol) + 1X TBE


DNA electrophoresis.  Separation of linear, double-stranded DNA, 80-500 bp

Acrylamide (29:1) - TBE

Composition 

Concentration Ingredient
3.5 % (wt:vol)

Acrylamide 29:1
1 X

TBE
0.07 % (wt:vol)

APS
0.035 % (vol:vol)

TEMED

Note 

Efficient range of separation for linear DNA molecules: 80 to 500 bp.  Migration of Xylene Cyanol FF: 260 bp.  Migration of Bromophenol Blue: 65 bp.

Calculator 

10
Amount to add Ingredient
1166.7 ul Acrylamide 29:1, 30% (wt:vol)
2 ml TBE, 5X
70.0 ul Ammonium Persulfate, 10% (wt:vol)
3.5 ul TEMED, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 68