Acrylamide Gel, 3.5% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 1000-2000 bp

Acrylamide (29:1) - TBE

Composition

Concentration	Ingredient
3.5 % (wt:vol)	Acrylamide 29:1
1 X	TBE
0.07 % (wt:vol)	APS
0.035 % (vol:vol)	TEMED

Note

Efficient range of separation for linear DNA molecules: 1000 to 2000 bp. Migration of Xylene Cyanol FF: 460 bp. Migration of Bromophenol Blue: 100 bp.

Calculator

Amount to add	Ingredient
1750.0 ul	Acrylamide 29:1, 30% (wt:vol)
3 ml	TBE, 5X
105.0 ul	Ammonium Persulfate, 10% (wt:vol)
5.3 ul	TEMED, 100% (vol:vol)

Reference(s)

Reference	Title	PMID/ISBN	Note
Roskams and Rodgers (eds.) 2002	Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench	9780879696306	p. 68