Acrylamide Gel, 15.0% (wt:vol) + 1X TBE


DNA electrophoresis.  Separation of linear, double-stranded DNA, 25-150 bp

Acrylamide (29:1) - TBE

Composition 

Concentration Ingredient
15 % (wt:vol)

Acrylamide 29:1

1 X

TBE

0.07 % (wt:vol)

APS

0.035 % (vol:vol)

TEMED

Note 

Efficient range of separation for linear DNA molecules: 25 to 150 bp.  Migration of Xylene Cyanol FF: 60 bp.  Migration of Bromophenol Blue: 15 bp.

Calculator 

1000
Amount to add Ingredient
500.0 ml Acrylamide 29:1, 30% (wt:vol)
200 ml TBE, 5X
7.0 ml Ammonium Persulfate, 10% (wt:vol)
350.0 ul TEMED, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 68