Acrylamide Gel, 12.0% (wt:vol) + 1X TBE


DNA electrophoresis.  Separation of linear, double-stranded DNA, 40-200 bp

Acrylamide (29:1) - TBE

Composition 

Concentration Ingredient
12 % (wt:vol)

Acrylamide 29:1
1 X

TBE
0.07 % (wt:vol)

APS
0.035 % (vol:vol)

TEMED

Note 

Efficient range of separation for linear DNA molecules: 40 to 200 bp.  Migration of Xylene Cyanol FF: 70 bp.  Migration of Bromophenol Blue: 20 bp.

Calculator 

12
Amount to add Ingredient
4.8 ml Acrylamide 29:1, 30% (wt:vol)
2.4 ml TBE, 5X
84.0 ul Ammonium Persulfate, 10% (wt:vol)
4.2 ul TEMED, 100% (vol:vol)


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 68