Acidic Tyrode Solution for Removing Zonae


Removal of zonae from mouse embryos

NaCl - KCl - CaCl2 - MgCl2 - Glucose - PVP

Composition 

Concentration Ingredient
8 g/l

NaCl
0.2 g/l

KCl
0.2 g/l

CaCl2

Dihydrate
0.1 g/l

MgCl2

Hexahydrate
1 g/l

Glucose
4 g/l

PVP 40

Note 

Adjust pH to 2.5 with HCl

Calculator 

250
Amount to add Ingredient
2.00 g Sodium Chloride (solid)
50.0 mg Potassium Chloride (solid)
50.0 mg Calcium Chloride Dihydrate (solid)
25.0 mg Magnesium Chloride Hexahydrate (solid)
250.0 mg Glucose (solid)
1.00 g Polyvinylpyrrolidone 40 (solid)


Outside Source for Solution
Vendor Grade Catalog No.
Sigma-Aldrich Suitable for mouse embryo T1788
Reference(s)
Reference Title PMID/ISBN Note
Mellick and Rodgers (eds.) 2006 Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench 9780879698157 p. 108