Acidic Stripping Buffer


Removal of antibodies from Western blots before incubation with another antibody

Glycine pH 2.1 - TWEEN 20 - β-mercaptoethanol

Composition 

Concentration Ingredient Details
100 mM

Glycine pH 2.1
0.5 % (vol:vol)

TWEEN® 20

 Original recipe specifies 0.5% (wt:vol) instead of (vol:vol). % (vol:vol) is a more common stock solution.
100 mM

β-mercaptoethanol

Note 

Adjust pH to 2.1 with HCl

Calculator 

10
Amount to add Ingredient Special Instructions
500.0 ul Glycine pH 2.1, 2 M
500.0 ul TWEEN® 20, 10% (vol:vol)
69.4 ul β-Mercaptoethanol, 14.4 M Add just before use


Reference(s)
Reference Title PMID/ISBN Note
Greenfield (ed.) 2014 Antibodies, A Laboratory Manual, 2nd Edition 9781936113811 p. 528