6% Acrylamide/Urea Top Solution


DNA Sequencing

Acrylamide - TBE - Urea

Composition 

Concentration Ingredient
6 % (wt:vol)

Acrylamide 19:1
1 X

TBE
46 % (wt:vol)

Urea

Calculator 

1000
Amount to add Ingredient
150.0 ml Acrylamide 19:1, 40% (wt:vol)
200 ml TBE, 5X
460.00 g Urea (solid)
Storage
Temperature
  • 4°C
Precaution(s)
  • Protected from light
Estimated Shelf Life
  • 1 month


Reference(s)
Reference Title PMID/ISBN Note
Roskams and Rodgers (eds.) 2002 Lab Ref, Volume 1: A Handbook of Recipes Reagents, and Other Reference Tools for Use at the Bench 9780879696306 p. 69