5% Formaldehyde


Preparation and immunolabeling of C. elegans

Paraformaldehyde - NaOH - PBS

Composition 

Concentration Ingredient
5 % (wt:vol)

Paraformaldehyde
1.6 mM

NaOH
1 X

PBS

Note 

Add Paraformaldehyde to water.  Add NaOH.  Heat at 65°C until dissolved. Filter and store at 4°C for up to 1 week. 

Calculator 

20
Amount to add Ingredient
1.00 g Paraformaldehyde (solid)
8.0 ul Sodium Hydroxide, 4 M
2 ml PBS, 10 X
Storage
Temperature
  • 4°C
Estimated Shelf Life
  • 1 week


Reference(s)
Reference Title PMID/ISBN Note
Spector and Goldman (eds.) 2006 Basic Methods in Microscopy 0879697512 p. 195