4% Paraformaldehyde

Preparation of embryos for live cell imaging

Paraformaldehyde - Sodium Phosphate pH 7.4

Composition

Concentration	Ingredient
4 % (wt:vol)	Paraformaldehyde
100 mM	Sodium Phosphate Buffer pH 7.4

Heat and stir at 60°C until clear. Adjust pH to 7.4 as needed.

Amount to add	Ingredient
40.00 g	Paraformaldehyde (solid)
100.0 ml	Sodium Phosphate Buffer pH 7.4, 1 M

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 40