4.0% Paraformaldehyde Stain


Fixation for imaging

PBS - Paraformaldehyde - MgCl2 - EGTA

Composition 

Concentration Ingredient
1 X

PBS
4 % (wt:vol)

Paraformaldehyde
2 mM

MgCl2
1.25 mM

EGTA pH 7.0

Calculator 

10
Amount to add Ingredient
1 ml PBS, 10 X
400.0 mg Paraformaldehyde (solid)
20.0 ul Magnesium Chloride, 1 M
12.5 ul EGTA pH 7.0, 1 M