1% Agarose in SDS Running Buffer | Nectagen

1% Agarose in SDS Running Buffer

Identification of novel protein kinase substrates

Agarose - Tris Base - Glycine - SDS

Composition

Concentration	Ingredient
25 mM	Tris Base
192 mM	Glycine
0.1 % (wt:vol)	SDS
1 % (wt:vol)	Agarose
0.002 % (wt:vol)	Bromophenol Blue

Calculator

Amount to add	Ingredient
302.9 mg	Tris Base (solid)
1.44 g	Glycine (solid)
100.0 mg	SDS (solid)
1.00 g	Agarose - Electrophoresis Grade
200.0 ul	Bromophenol Blue, 1% (wt:vol)

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 83