0.25% Trypsin in Tris-Saline for Tissue Culture | Nectagen

0.25% Trypsin in Tris-Saline for Tissue Culture

Dissociation of cells and embryos

Trypsin - NaCl - KCl - Na₂HPO₄ - Glucose - Tris Base - Phenol Red - Penicillin G - Streptomycin

Composition

Concentration	Ingredient
8 g/l	NaCl
0.4 g/l	KCl
0.1 g/l	Na₂HPO₄
1 g/l	Glucose
3 g/l	Tris Base
0.01 g/l	Phenol Red
60 ug/ml	Penicillin G Potassium Salt
100 ug/ml	Streptomycin Sulfate Salt
2.5 g/l	Trypsin - Bovine Pancreas

Note

Adjust pH to 7.6.

Calculator

Amount to add	Ingredient	Special Instructions
8.0 mg	Sodium Chloride (solid)
0.4 mg	Potassium Chloride (solid)
0.1 mg	Sodium Phosphate Dibasic Anhydrous (solid)
1.0 mg	Glucose (solid)
3.0 mg	Tris Base (solid)
0.0 mg	Phenol Red (solid)
0.6 ul	Penicillin G Potassium Salt, 100 mg/ml
5.0 ul	Streptomycin Sulfate Salt, 20 mg/ml
2.5 mg	Trypsin - Bovine Pancreas (solid)	Dissolve in small volume of water before adding

Storage
Temperature	-20°C

Reference(s)

Reference	Title	PMID/ISBN	Note
Mellick and Rodgers (eds.) 2006	Lab Ref, Volume 2: A Handbook of Recipes, Reagents and Other Reference Tools for Use at the Bench	9780879698157	p. 111