Solutions
Name | Use | Description |
---|---|---|
0.25% Trypsin in Tris-Saline for Tissue Culture |
Dissociation of cells and embryos |
Trypsin - NaCl - KCl - Na2HPO4 - Glucose - Tris Base - Phenol Red - Penicillin G - Streptomycin |
1% Agarose in SDS Running Buffer |
Identification of novel protein kinase substrates |
Agarose - Tris Base - Glycine - SDS |
2% M-PBS-T |
Phosphate buffered saline with 2% non-fat dry milk and 0.05% Tween 20 |
|
20% PEG 8000 2.5 M NaCl |
Precipitation of phage |
PEG - NaCl |
2X YT |
Rich liquid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl |
2X YT - 0.1% Glucose |
Rich liquid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose |
2X YT - 1% Glucose |
Rich liquid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose |
2X YT - 100 ug/ml Carbenecillin |
Rich solid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin |
2X YT - 2% Glucose |
Rich liquid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose |
2X YT - 2% Glucose - 100 ug/ml Carbenecillin |
Rich solid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin |
2X YT Agar Plates - 2% Glucose |
Rich solid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose - Agar |
2X YT Agar Plates - 2% Glucose - 100 ug/ml Carbenecillin |
Rich solid medium for bacterial culture |
Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin |
4% M-PBS-T |
Blocking agent |
PBS - TWEEN 20 - Milk |
4% Paraformaldehyde |
Preparation of embryos for live cell imaging |
Paraformaldehyde - Sodium Phosphate pH 7.4 |
4.0% Paraformaldehyde Stain |
Fixation for imaging |
PBS - Paraformaldehyde - MgCl2 - EGTA |
5% Formaldehyde |
Preparation and immunolabeling of C. elegans |
Paraformaldehyde - NaOH - PBS |
50% PEG 3350 Lithium Acetate pH 4.9 |
PEG 3350 - Lithium Acetate |
|
6% Acrylamide/Urea Top Solution |
DNA Sequencing |
Acrylamide - TBE - Urea |
Acetic Acid Fix and Stop Solution |
Mass spectrometry |
Ethanol - Acetic Acid |
Acetylation Mix |
Peptide arrays |
Acetic Anhydride - DMF |
Acid Citrate Dextrose (ACD) |
Isolation of total DNA from blood |
Citric Acid - Sodium Citrate - Glucose |
Acid Precipitation Solution |
Precipitation of nucleic acids |
HCl - Sodium Pyrophosphate |
Acidic Stripping Buffer |
Removal of antibodies from Western blots before incubation with another antibody |
Glycine pH 2.1 - TWEEN 20 - β-mercaptoethanol |
Acidic Tyrode Solution for Removing Zonae |
Removal of zonae from mouse embryos |
NaCl - KCl - CaCl2 - MgCl2 - Glucose - PVP |
Acridine Orange Staining Solution |
Cell staining for Dynal T4 Quant |
|
Acrylamide 19:1, 40% (wt:vol) |
Polyacrylamide gel electrophoresis for sequencing DNA and separating peptides |
19:1 Acrylamide - Bis-acrylamide |
Acrylamide 29:1, 30% (wt:vol) |
Polyacrylamide gel electrophoresis for sequencing DNA and separating proteins |
29:1 Acrylamide - Bis-acrylamide |
Acrylamide 37.5:1, 40% (wt:vol) |
SDS polyacrylamide gel electrophoresis of high molecular weight proteins |
37.5:1 Acrylamide - Bis-acrylamide |
Acrylamide Gel, 12.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 40-200 bp |
Acrylamide (29:1) - TBE |
Acrylamide Gel, 15.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 25-150 bp |
Acrylamide (29:1) - TBE |
Acrylamide Gel, 20.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 6-100 bp |
Acrylamide (29:1) - TBE |
Acrylamide Gel, 3.5% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 1000-2000 bp |
Acrylamide (29:1) - TBE |
Acrylamide Gel, 5.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 80-500 bp |
Acrylamide (29:1) - TBE |
Acrylamide Gel, 8.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 60-400 bp |
Acrylamide (29:1) - TBE |
AE Buffer |
Isolation of total RNA from yeast |
Sodium Acetate pH 6.0 - EDTA |
Agar Sucrose (ZFIN) | ||
Agarose Gel, 0.3% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb |
Agarose - TBE |
Agarose Gel, 0.3% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb |
Agarose - TBE |
Agarose Gel, 0.6% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb |
Agarose - TBE |
Agarose Gel, 0.6% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb |
Agarose - TBE |
Agarose Gel, 0.7% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb |
Agarose - TBE |
Agarose Gel, 0.7% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb |
Agarose - TBE |
Agarose Gel, 0.9% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7 |
Agarose - TBE |
Agarose Gel, 0.9% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7 kb |
Agarose - TBE |
Agarose Gel, 1.2% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb |
Agarose - TBE |
Agarose Gel, 1.2% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb |
Agarose - TBE |
Agarose Gel, 1.5% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb |
Agarose - TBE |
Agarose Gel, 1.5% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb |
Agarose - TBE |
Agarose Gel, 2.0% (wt:vol) + 0.5X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb |
Agarose - TBE |
Agarose Gel, 2.0% (wt:vol) + 1X TBE |
DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb |
Agarose - TBE |
AHC Agar |
Solid media for S. cerevisiae |
YNB - Casein Acid Hydrolysate - Tryptophan |
AHC Medium |
Media for S. cerevisiae |
YNB - Casein Acid Hydrolysate - Adenine |
AHC Medium + Trp |
Media for Trp- S. cerevisiae |
YNB - Casein Acid Hydrolysate - Tryptophan |
AHC Medium - High Adenine |
Media for Ade- S. cerevisiae |
YNB - Casein Acid Hydrolysate - Adenine |
Alkaline Loading Buffer, 6X |
Loading dye for electrophoresis |
NaOH - EDTA - Ficoll Bromocresol Green - Xylene Cyanol FF |
Alkaline Lysis Plasmid Miniprep Solution I |
Minpreparation of plasmid DNA from E. coli |
Glucose - Tris-Cl pH 8.0 - EDTA |
Alkaline Lysis Plasmid Miniprep Solution II |
Minpreparation of plasmid DNA from E. coli |
NaOH - SDS |
Alkaline Lysis Plasmid Miniprep Solution III |
Minpreparation of plasmid DNA from E. coli |
Potassium Acetate - Acetic Acid |
Alkaline Phosphatase Buffer |
Detection of alkaline phosphatase activity |
NaCl - Tris-Cl pH 9.5 - MgCl2 |
Alkaline Phosphatase Staining Solution |
Alkaline phosphatase staining of mouse embryos |
Tris-Maleate pH 9.0 - α-Naphthyl Phosphate Disodium Salt - Fast Red TR - MgCl2 |
Alkaline Running Buffer |
RNA electrophoresis running buffer |
NaOH - EDTA |
Ammonium Persulfate (10%) |
Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis |
Ammonium Persulfate - Phosphoric Acid - Sodium Hydroxide |
Ammonium Sulfate Cut Dilution Buffer (ASCDB) |
Purification of proteins and inhibitors from cell culture |
Tris-Cl pH 7.2 - DTT |
Ampholyte Mixture |
Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis |
Ampholyte (pH 6.0-8.0 or other narrow range) - Ampholyte (pH 3.5-10.0 or other wide range) |
Amplification Buffer, 10X |
Isolation and amplification of probes for yeast DNA microarrays |
KCl - Tris-Cl pH 8.3 |
AMV-RT, 10X |
Isolation and amplification of RNA from single cells and small amounts of tissue |
Tris-Cl pH 8.3 - KCl - MgCl2 |
Annealing Buffer, 10X |
Nuclease S1 RNA protection |
Tris-Cl pH 7.5 - MgCl2 - NaCl - DTT |
Annealing Buffer, 2X |
RNAi in mammalians |
Potassium Acetate - Magnesium Acetate - HEPES-KOH pH 7.4 |
Antigen Dialysis Buffer |
Electroelution of protein antigens from polyacrylamide gel slices |
Tris-Acetate pH 7.4 - SDS - DTT |
Antigen Electrophoresis Buffer |
Electroelution of protein antigens from polyacrylamide gel slices |
Tris-Acetate pH 7.4 - SDS - Sodium Thioglycolate |
AP 7.5 |
Detection of probes labeled with alkaline phosphatase |
Tris-Cl pH 7.5 - NaCl - MgCl2 |
AP 9.5 |
Detection of probes labeled with alkaline phosphatase |
Tris-Cl pH 9.5 - NaCl - MgCl2 |
Apple Juice Agar Plate |
Collection of Drosophila embryos |
Agar - Sucrose - Apple Juice - Methyl-4-hydroxybenzoate |
Apple Plate |
Collection of Drosophila embryos |
Apple Juice - Agar |
Artificial Cerebrospinal Fluid |
Artitifical Cerebrospinal Fluid |
NaCl - KCl - MgSO4 - KH2PO4 - NaHCO3 - Glucose - Sucrose - CaCl2 |
Assembly Buffer |
Imaging intermediate filament proteins in living cells |
Sodium/Potassium Phosphate pH 7.4 - NaCl - KCl - β-mercaptoethanol - PMSF |
BABB |
Immunohistochemistry of whole-mount mouse embryos |
Benzyl Alcohol - Benzyl Benzoate |
Basic Buffer |
Use and labeling of GST fusions |
HEPES pH 7.15 - KCl - MgCl2 - DTT - IGEPAL CA-630 |
BBBA |
Benzyl Benzoate:Benzyl Alcohol |
|
BBS, 2X |
Cytoskeleton visualization |
NaCl - Sodium Phosphate Dibasic - BES pH 7.1 |
BCA Reagent A |
Component of BCA protein concentration assay |
Bicinchoninic Acid - Na2CO3 - Sodium Tartrate - NaOH - NaHCO3 |
BCA Reagent B |
Component of BCA protein concentration assay |
CuSO4 |
Binding Buffer - IMAC |
Immobilized metal-ion affinity chromatography |
Sodium Phosphate pH 7.4 - NaCl - Low Imididazole |
Binding Buffer - Phage Display |
Phage display |
Tris-Cl pH 7.5 - KCl - MgCl2 - DTT - Glycerol - Milk - Tween-20 . Pefabloc SC - Benzamidine - Aprotinin - Leupeptin - NaF - Na3VO4 |
Biocytin 0.5% (wt:vol) in Pipette Solution |
Ballistic delivery of dyes to neurons |
Biocytin - Pipette Solution |
Bleaching Solution (Xenopus) |
Immunohistochemistry with Xenopus |
H2O2 - Formamide - SSC |
Bleaching Solution (ZFIN) | ||
Blocking Buffer - Phage Display |
Phage display |
PBS - BSA |
Blocking Buffer for Luminescence Detection |
Tris-Buffered Saline pH 7.5 |
|
Blocking Solution - Phage Display |
Phage display |
PBS - Milk - PMSF - Benzamidine |
BLOTTO |
Blocking solution for Western blotting |
Nonfat Dried Milk - Antifoam A - Sodium Azide - PBS |
Blunt-end Ligation Buffer, 10X |
Blunt-end ligation of DNA fragments |
Tris-Cl pH 7.6 - MgCl2 - DTT - BSA - Hexamminecobalt Chloride - ATP - Spermidine HCL |
Bodian's No. 2 Fixative |
Sample fixation |
Formaldehyde - Acetic Acid - Ethanol |
Bone Staining Solution (ZFIN) | ||
Bouin's Fixative |
Preparation of parrafin tissue sections |
Dissolve picric acid in a volume of water equal to half of final volume. Filter through Whatman No. 1 paper or equivalent. Add paraformaldehyde and heat to 60C in a fume hood. Add a few drops of 1 N NaOH to help dissolution. Cool to room temperature and add remaining ingredients. |
BPTE Electrophoresis Buffer, 10X |
Hybridization analysis of nucleic acids |
PIPES - Bis-Tris - EDTA |
Bradford Reagent |
Protein concentration assay |
Coomassie Brilliant Blue G-250 - Ethanol - Phosphoric Acid |
BTE |
Purification of high molecular weight DNA from plant cells |
Tris-Cl pH 8.0 - EDTA |
Buffer 1 - Calorimetry |
Calorimetry analysis of protein-protein interactions |
Tris-Cl pH 8.0 - MgCl2 |
Buffer 2 - Calorimetry |
Calorimetry analysis of protein-protein interactions |
Tris-Cl pH 8.0 - MgCl2 |