Solutions

Name	Use	Description
0.25% Trypsin in Tris-Saline for Tissue Culture	Dissociation of cells and embryos	Trypsin - NaCl - KCl - Na₂HPO₄ - Glucose - Tris Base - Phenol Red - Penicillin G - Streptomycin
1% Agarose in SDS Running Buffer	Identification of novel protein kinase substrates	Agarose - Tris Base - Glycine - SDS
2% M-PBS-T		Phosphate buffered saline with 2% non-fat dry milk and 0.05% Tween 20
20% PEG 8000 2.5 M NaCl	Precipitation of phage	PEG - NaCl
2X YT	Rich liquid medium for bacterial culture	Yeast Extract - Tryptone - NaCl
2X YT - 0.1% Glucose	Rich liquid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose
2X YT - 1% Glucose	Rich liquid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose
2X YT - 100 ug/ml Carbenecillin	Rich solid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin
2X YT - 2% Glucose	Rich liquid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose
2X YT - 2% Glucose - 100 ug/ml Carbenecillin	Rich solid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin
2X YT Agar Plates - 2% Glucose	Rich solid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose - Agar
2X YT Agar Plates - 2% Glucose - 100 ug/ml Carbenecillin	Rich solid medium for bacterial culture	Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin
4% M-PBS-T	Blocking agent	PBS - TWEEN 20 - Milk
4% Paraformaldehyde	Preparation of embryos for live cell imaging	Paraformaldehyde - Sodium Phosphate pH 7.4
4.0% Paraformaldehyde Stain	Fixation for imaging	PBS - Paraformaldehyde - MgCl₂ - EGTA
5% Formaldehyde	Preparation and immunolabeling of C. elegans	Paraformaldehyde - NaOH - PBS
50% PEG 3350 Lithium Acetate pH 4.9		PEG 3350 - Lithium Acetate
6% Acrylamide/Urea Top Solution	DNA Sequencing	Acrylamide - TBE - Urea
Acetic Acid Fix and Stop Solution	Mass spectrometry	Ethanol - Acetic Acid
Acetylation Mix	Peptide arrays	Acetic Anhydride - DMF
Acid Citrate Dextrose (ACD)	Isolation of total DNA from blood	Citric Acid - Sodium Citrate - Glucose
Acid Precipitation Solution	Precipitation of nucleic acids	HCl - Sodium Pyrophosphate
Acidic Stripping Buffer	Removal of antibodies from Western blots before incubation with another antibody	Glycine pH 2.1 - TWEEN 20 - β-mercaptoethanol
Acidic Tyrode Solution for Removing Zonae	Removal of zonae from mouse embryos	NaCl - KCl - CaCl₂ - MgCl₂ - Glucose - PVP
Acridine Orange Staining Solution	Cell staining for Dynal T4 Quant
Acrylamide 19:1, 40% (wt:vol)	Polyacrylamide gel electrophoresis for sequencing DNA and separating peptides	19:1 Acrylamide - Bis-acrylamide
Acrylamide 29:1, 30% (wt:vol)	Polyacrylamide gel electrophoresis for sequencing DNA and separating proteins	29:1 Acrylamide - Bis-acrylamide
Acrylamide 37.5:1, 40% (wt:vol)	SDS polyacrylamide gel electrophoresis of high molecular weight proteins	37.5:1 Acrylamide - Bis-acrylamide
Acrylamide Gel, 12.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 40-200 bp	Acrylamide (29:1) - TBE
Acrylamide Gel, 15.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 25-150 bp	Acrylamide (29:1) - TBE
Acrylamide Gel, 20.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 6-100 bp	Acrylamide (29:1) - TBE
Acrylamide Gel, 3.5% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 1000-2000 bp	Acrylamide (29:1) - TBE
Acrylamide Gel, 5.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 80-500 bp	Acrylamide (29:1) - TBE
Acrylamide Gel, 8.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 60-400 bp	Acrylamide (29:1) - TBE
AE Buffer	Isolation of total RNA from yeast	Sodium Acetate pH 6.0 - EDTA
Agar Sucrose (ZFIN)
Agarose Gel, 0.3% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb	Agarose - TBE
Agarose Gel, 0.3% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb	Agarose - TBE
Agarose Gel, 0.6% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb	Agarose - TBE
Agarose Gel, 0.6% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb	Agarose - TBE
Agarose Gel, 0.7% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb	Agarose - TBE
Agarose Gel, 0.7% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb	Agarose - TBE
Agarose Gel, 0.9% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7	Agarose - TBE
Agarose Gel, 0.9% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7 kb	Agarose - TBE
Agarose Gel, 1.2% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb	Agarose - TBE
Agarose Gel, 1.2% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb	Agarose - TBE
Agarose Gel, 1.5% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb	Agarose - TBE
Agarose Gel, 1.5% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb	Agarose - TBE
Agarose Gel, 2.0% (wt:vol) + 0.5X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb	Agarose - TBE
Agarose Gel, 2.0% (wt:vol) + 1X TBE	DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb	Agarose - TBE
AHC Agar	Solid media for S. cerevisiae	YNB - Casein Acid Hydrolysate - Tryptophan
AHC Medium	Media for S. cerevisiae	YNB - Casein Acid Hydrolysate - Adenine
AHC Medium + Trp	Media for Trp- S. cerevisiae	YNB - Casein Acid Hydrolysate - Tryptophan
AHC Medium - High Adenine	Media for Ade- S. cerevisiae	YNB - Casein Acid Hydrolysate - Adenine
Alkaline Loading Buffer, 6X	Loading dye for electrophoresis	NaOH - EDTA - Ficoll Bromocresol Green - Xylene Cyanol FF
Alkaline Lysis Plasmid Miniprep Solution I	Minpreparation of plasmid DNA from E. coli	Glucose - Tris-Cl pH 8.0 - EDTA
Alkaline Lysis Plasmid Miniprep Solution II	Minpreparation of plasmid DNA from E. coli	NaOH - SDS
Alkaline Lysis Plasmid Miniprep Solution III	Minpreparation of plasmid DNA from E. coli	Potassium Acetate - Acetic Acid
Alkaline Phosphatase Buffer	Detection of alkaline phosphatase activity	NaCl - Tris-Cl pH 9.5 - MgCl₂
Alkaline Phosphatase Staining Solution	Alkaline phosphatase staining of mouse embryos	Tris-Maleate pH 9.0 - α-Naphthyl Phosphate Disodium Salt - Fast Red TR - MgCl2
Alkaline Running Buffer	RNA electrophoresis running buffer	NaOH - EDTA
Ammonium Persulfate (10%)	Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis	Ammonium Persulfate - Phosphoric Acid - Sodium Hydroxide
Ammonium Sulfate Cut Dilution Buffer (ASCDB)	Purification of proteins and inhibitors from cell culture	Tris-Cl pH 7.2 - DTT
Ampholyte Mixture	Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis	Ampholyte (pH 6.0-8.0 or other narrow range) - Ampholyte (pH 3.5-10.0 or other wide range)
Amplification Buffer, 10X	Isolation and amplification of probes for yeast DNA microarrays	KCl - Tris-Cl pH 8.3
AMV-RT, 10X	Isolation and amplification of RNA from single cells and small amounts of tissue	Tris-Cl pH 8.3 - KCl - MgCl₂
Annealing Buffer, 10X	Nuclease S1 RNA protection	Tris-Cl pH 7.5 - MgCl₂ - NaCl - DTT
Annealing Buffer, 2X	RNAi in mammalians	Potassium Acetate - Magnesium Acetate - HEPES-KOH pH 7.4
Antigen Dialysis Buffer	Electroelution of protein antigens from polyacrylamide gel slices	Tris-Acetate pH 7.4 - SDS - DTT
Antigen Electrophoresis Buffer	Electroelution of protein antigens from polyacrylamide gel slices	Tris-Acetate pH 7.4 - SDS - Sodium Thioglycolate
AP 7.5	Detection of probes labeled with alkaline phosphatase	Tris-Cl pH 7.5 - NaCl - MgCl2
AP 9.5	Detection of probes labeled with alkaline phosphatase	Tris-Cl pH 9.5 - NaCl - MgCl2
Apple Juice Agar Plate	Collection of Drosophila embryos	Agar - Sucrose - Apple Juice - Methyl-4-hydroxybenzoate
Apple Plate	Collection of Drosophila embryos	Apple Juice - Agar
Artificial Cerebrospinal Fluid	Artitifical Cerebrospinal Fluid	NaCl - KCl - MgSO₄ - KH₂PO₄ - NaHCO₃ - Glucose - Sucrose - CaCl₂
Assembly Buffer	Imaging intermediate filament proteins in living cells	Sodium/Potassium Phosphate pH 7.4 - NaCl - KCl - β-mercaptoethanol - PMSF
BABB	Immunohistochemistry of whole-mount mouse embryos	Benzyl Alcohol - Benzyl Benzoate
Basic Buffer	Use and labeling of GST fusions	HEPES pH 7.15 - KCl - MgCl₂ - DTT - IGEPAL CA-630
BBBA		Benzyl Benzoate:Benzyl Alcohol
BBS, 2X	Cytoskeleton visualization	NaCl - Sodium Phosphate Dibasic - BES pH 7.1
BCA Reagent A	Component of BCA protein concentration assay	Bicinchoninic Acid - Na₂CO₃ - Sodium Tartrate - NaOH - NaHCO₃
BCA Reagent B	Component of BCA protein concentration assay	CuSO₄
Binding Buffer - IMAC	Immobilized metal-ion affinity chromatography	Sodium Phosphate pH 7.4 - NaCl - Low Imididazole
Binding Buffer - Phage Display	Phage display	Tris-Cl pH 7.5 - KCl - MgCl₂ - DTT - Glycerol - Milk - Tween-20 . Pefabloc SC - Benzamidine - Aprotinin - Leupeptin - NaF - Na₃VO₄
Biocytin 0.5% (wt:vol) in Pipette Solution	Ballistic delivery of dyes to neurons	Biocytin - Pipette Solution
Bleaching Solution (Xenopus)	Immunohistochemistry with Xenopus	H2O2 - Formamide - SSC
Bleaching Solution (ZFIN)
Blocking Buffer - Phage Display	Phage display	PBS - BSA
Blocking Buffer for Luminescence Detection		Tris-Buffered Saline pH 7.5
Blocking Solution - Phage Display	Phage display	PBS - Milk - PMSF - Benzamidine
BLOTTO	Blocking solution for Western blotting	Nonfat Dried Milk - Antifoam A - Sodium Azide - PBS
Blunt-end Ligation Buffer, 10X	Blunt-end ligation of DNA fragments	Tris-Cl pH 7.6 - MgCl2 - DTT - BSA - Hexamminecobalt Chloride - ATP - Spermidine HCL
Bodian's No. 2 Fixative	Sample fixation	Formaldehyde - Acetic Acid - Ethanol
Bone Staining Solution (ZFIN)
Bouin's Fixative	Preparation of parrafin tissue sections	Dissolve picric acid in a volume of water equal to half of final volume. Filter through Whatman No. 1 paper or equivalent. Add paraformaldehyde and heat to 60C in a fume hood. Add a few drops of 1 N NaOH to help dissolution. Cool to room temperature and add remaining ingredients.
BPTE Electrophoresis Buffer, 10X	Hybridization analysis of nucleic acids	PIPES - Bis-Tris - EDTA
Bradford Reagent	Protein concentration assay	Coomassie Brilliant Blue G-250 - Ethanol - Phosphoric Acid
BTE	Purification of high molecular weight DNA from plant cells	Tris-Cl pH 8.0 - EDTA
Buffer 1 - Calorimetry	Calorimetry analysis of protein-protein interactions	Tris-Cl pH 8.0 - MgCl₂
Buffer 2 - Calorimetry	Calorimetry analysis of protein-protein interactions	Tris-Cl pH 8.0 - MgCl₂

Nectagen

Solutions

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