Solutions

Name Use Description
0.25% Trypsin in Tris-Saline for Tissue Culture

Dissociation of cells and embryos

Trypsin - NaCl - KCl - Na2HPO4 - Glucose - Tris Base - Phenol Red - Penicillin G - Streptomycin

1% Agarose in SDS Running Buffer

Identification of novel protein kinase substrates

Agarose - Tris Base - Glycine - SDS 

2% M-PBS-T

Phosphate buffered saline with 2% non-fat dry milk and 0.05% Tween 20

20% PEG 8000 2.5 M NaCl

Precipitation of phage

PEG - NaCl

2X YT

Rich liquid medium for bacterial culture

Yeast Extract - Tryptone - NaCl

2X YT - 0.1% Glucose

Rich liquid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose

2X YT - 1% Glucose

Rich liquid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose

2X YT - 100 ug/ml Carbenecillin

Rich solid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin

2X YT - 2% Glucose

Rich liquid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose

2X YT - 2% Glucose - 100 ug/ml Carbenecillin

Rich solid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin

2X YT Agar Plates - 2% Glucose

Rich solid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose - Agar

2X YT Agar Plates - 2% Glucose - 100 ug/ml Carbenecillin

Rich solid medium for bacterial culture

Yeast Extract - Tryptone - NaCl - Glucose - Agar - Carbenecillin

4% M-PBS-T

Blocking agent

PBS - TWEEN 20 - Milk

4% Paraformaldehyde

Preparation of embryos for live cell imaging

Paraformaldehyde - Sodium Phosphate pH 7.4

4.0% Paraformaldehyde Stain

Fixation for imaging

PBS - Paraformaldehyde - MgCl2 - EGTA

5% Formaldehyde

Preparation and immunolabeling of C. elegans

Paraformaldehyde - NaOH - PBS

50% PEG 3350 Lithium Acetate pH 4.9

PEG 3350 - Lithium Acetate

6% Acrylamide/Urea Top Solution

DNA Sequencing

Acrylamide - TBE - Urea

Acetic Acid Fix and Stop Solution

Mass spectrometry

Ethanol - Acetic Acid

Acetylation Mix

Peptide arrays

Acetic Anhydride - DMF

Acid Citrate Dextrose (ACD)

Isolation of total DNA from blood

Citric Acid - Sodium Citrate - Glucose

Acid Precipitation Solution

Precipitation of nucleic acids

HCl - Sodium Pyrophosphate

Acidic Stripping Buffer

Removal of antibodies from Western blots before incubation with another antibody

Glycine pH 2.1 - TWEEN 20 - β-mercaptoethanol

Acidic Tyrode Solution for Removing Zonae

Removal of zonae from mouse embryos

NaCl - KCl - CaCl2 - MgCl2 - Glucose - PVP

Acridine Orange Staining Solution

Cell staining for Dynal T4 Quant

Acrylamide 19:1, 40% (wt:vol)

Polyacrylamide gel electrophoresis for sequencing DNA and separating peptides

19:1 Acrylamide - Bis-acrylamide

Acrylamide 29:1, 30% (wt:vol)

Polyacrylamide gel electrophoresis for sequencing DNA and separating proteins

29:1 Acrylamide - Bis-acrylamide

Acrylamide 37.5:1, 40% (wt:vol)

SDS polyacrylamide gel electrophoresis of high molecular weight proteins

37.5:1 Acrylamide - Bis-acrylamide

Acrylamide Gel, 12.0% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 40-200 bp

Acrylamide (29:1) - TBE

Acrylamide Gel, 15.0% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 25-150 bp

Acrylamide (29:1) - TBE

Acrylamide Gel, 20.0% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 6-100 bp

Acrylamide (29:1) - TBE

Acrylamide Gel, 3.5% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 1000-2000 bp

Acrylamide (29:1) - TBE

Acrylamide Gel, 5.0% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 80-500 bp

Acrylamide (29:1) - TBE

Acrylamide Gel, 8.0% (wt:vol) + 1X TBE

DNA electrophoresis.  Separation of linear, double-stranded DNA, 60-400 bp

Acrylamide (29:1) - TBE

AE Buffer

Isolation of total RNA from yeast

Sodium Acetate pH 6.0 - EDTA

Agar Sucrose (ZFIN)
Agarose Gel, 0.3% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb

Agarose - TBE

Agarose Gel, 0.3% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 5-60 kb

Agarose - TBE

Agarose Gel, 0.6% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb

Agarose - TBE

Agarose Gel, 0.6% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 1-20 kb

Agarose - TBE

Agarose Gel, 0.7% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb

Agarose - TBE

Agarose Gel, 0.7% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.8-10 kb

Agarose - TBE

Agarose Gel, 0.9% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7

Agarose - TBE

Agarose Gel, 0.9% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.5-7 kb

Agarose - TBE

Agarose Gel, 1.2% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb

Agarose - TBE

Agarose Gel, 1.2% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.4-6 kb

Agarose - TBE

Agarose Gel, 1.5% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb

Agarose - TBE

Agarose Gel, 1.5% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.2-3 kb

Agarose - TBE

Agarose Gel, 2.0% (wt:vol) + 0.5X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb

Agarose - TBE

Agarose Gel, 2.0% (wt:vol) + 1X TBE

DNA electrophoresis. Separation of linear, double-stranded DNA, 0.1-2 kb

Agarose - TBE

AHC Agar

Solid media for S. cerevisiae

YNB - Casein Acid Hydrolysate - Tryptophan

AHC Medium

Media for S. cerevisiae

YNB - Casein Acid Hydrolysate - Adenine

AHC Medium + Trp

Media for Trp- S. cerevisiae

YNB - Casein Acid Hydrolysate - Tryptophan

AHC Medium - High Adenine

Media for Ade- S. cerevisiae

YNB - Casein Acid Hydrolysate - Adenine

Alkaline Loading Buffer, 6X

Loading dye for electrophoresis

NaOH - EDTA - Ficoll Bromocresol Green - Xylene Cyanol FF

Alkaline Lysis Plasmid Miniprep Solution I

Minpreparation of plasmid DNA from E. coli

Glucose - Tris-Cl pH 8.0 - EDTA

Alkaline Lysis Plasmid Miniprep Solution II

Minpreparation of plasmid DNA from E. coli

NaOH - SDS

Alkaline Lysis Plasmid Miniprep Solution III

Minpreparation of plasmid DNA from E. coli

Potassium Acetate - Acetic Acid

Alkaline Phosphatase Buffer

Detection of alkaline phosphatase activity

NaCl - Tris-Cl pH 9.5 - MgCl2

Alkaline Phosphatase Staining Solution

Alkaline phosphatase staining of mouse embryos

Tris-Maleate pH 9.0 - α-Naphthyl Phosphate Disodium Salt - Fast Red TR - MgCl2

Alkaline Running Buffer

RNA electrophoresis running buffer

NaOH - EDTA

Ammonium Persulfate (10%)

Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis

Ammonium Persulfate - Phosphoric Acid - Sodium Hydroxide

Ammonium Sulfate Cut Dilution Buffer (ASCDB)

Purification of proteins and inhibitors from cell culture

Tris-Cl pH 7.2 - DTT

Ampholyte Mixture

Two-dimensional isoelectric focusing/SDS-polyacrylamide gel electrophoresis

Ampholyte (pH 6.0-8.0 or other narrow range) - Ampholyte (pH 3.5-10.0 or other wide range)

Amplification Buffer, 10X

Isolation and amplification of probes for yeast DNA microarrays

KCl - Tris-Cl pH 8.3

AMV-RT, 10X

Isolation and amplification of RNA from single cells and small amounts of tissue

Tris-Cl pH 8.3 - KCl - MgCl2

Annealing Buffer, 10X

Nuclease S1 RNA protection

Tris-Cl pH 7.5 - MgCl2 - NaCl - DTT

Annealing Buffer, 2X

RNAi in mammalians

Potassium Acetate - Magnesium Acetate - HEPES-KOH pH 7.4

Antigen Dialysis Buffer

Electroelution of protein antigens from polyacrylamide gel slices

Tris-Acetate pH 7.4 - SDS - DTT

Antigen Electrophoresis Buffer

Electroelution of protein antigens from polyacrylamide gel slices

Tris-Acetate pH 7.4 - SDS - Sodium Thioglycolate

AP 7.5

Detection of probes labeled with alkaline phosphatase

Tris-Cl pH 7.5 - NaCl - MgCl2

AP 9.5

Detection of probes labeled with alkaline phosphatase

Tris-Cl pH 9.5 - NaCl - MgCl2

Apple Juice Agar Plate

Collection of Drosophila embryos

Agar - Sucrose - Apple Juice - Methyl-4-hydroxybenzoate

Apple Plate

Collection of Drosophila embryos

Apple Juice - Agar

Artificial Cerebrospinal Fluid

Artitifical Cerebrospinal Fluid

NaCl - KCl - MgSO4 - KH2PO4 - NaHCO3 - Glucose - Sucrose - CaCl2

Assembly Buffer

Imaging intermediate filament proteins in living cells

Sodium/Potassium Phosphate pH 7.4 - NaCl - KCl - β-mercaptoethanol - PMSF

BABB

Immunohistochemistry of whole-mount mouse embryos

Benzyl Alcohol - Benzyl Benzoate

Basic Buffer

Use and labeling of GST fusions

HEPES pH 7.15 - KCl - MgCl2 - DTT - IGEPAL CA-630

BBBA

Benzyl Benzoate:Benzyl Alcohol

BBS, 2X

Cytoskeleton visualization

NaCl - Sodium Phosphate Dibasic - BES pH 7.1

BCA Reagent A

Component of BCA protein concentration assay

Bicinchoninic Acid - Na2CO3 - Sodium Tartrate - NaOH - NaHCO3

BCA Reagent B

Component of BCA protein concentration assay

CuSO4

Binding Buffer - IMAC

Immobilized metal-ion affinity chromatography

Sodium Phosphate pH 7.4 - NaCl - Low Imididazole

Binding Buffer - Phage Display

Phage display

Tris-Cl pH 7.5 - KCl - MgCl2 - DTT - Glycerol - Milk - Tween-20 . Pefabloc SC - Benzamidine - Aprotinin - Leupeptin - NaF - Na3VO4

Biocytin 0.5% (wt:vol) in Pipette Solution

Ballistic delivery of dyes to neurons

Biocytin - Pipette Solution

Bleaching Solution (Xenopus)

Immunohistochemistry with Xenopus

H2O2 - Formamide - SSC

Bleaching Solution (ZFIN)
Blocking Buffer - Phage Display

Phage display 

PBS - BSA

Blocking Buffer for Luminescence Detection

Tris-Buffered Saline pH 7.5

Blocking Solution - Phage Display

Phage display

PBS - Milk - PMSF - Benzamidine

BLOTTO

Blocking solution for Western blotting

Nonfat Dried Milk - Antifoam A - Sodium Azide - PBS

Blunt-end Ligation Buffer, 10X

Blunt-end ligation of DNA fragments

Tris-Cl pH 7.6 - MgCl2 - DTT - BSA - Hexamminecobalt Chloride - ATP - Spermidine HCL

Bodian's No. 2 Fixative

Sample fixation

Formaldehyde - Acetic Acid - Ethanol

Bone Staining Solution (ZFIN)
Bouin's Fixative

Preparation of parrafin tissue sections

Dissolve picric acid in a volume of water equal to half of final volume. Filter through Whatman No. 1 paper or equivalent.  Add paraformaldehyde and heat to 60C in a fume hood.  Add a few drops of 1 N NaOH to help dissolution.  Cool to room temperature and add remaining ingredients. 

BPTE Electrophoresis Buffer, 10X

Hybridization analysis of nucleic acids

PIPES - Bis-Tris - EDTA

Bradford Reagent

Protein concentration assay

Coomassie Brilliant Blue G-250 - Ethanol - Phosphoric Acid

BTE

Purification of high molecular weight DNA from plant cells

Tris-Cl pH 8.0 - EDTA

Buffer 1 - Calorimetry

Calorimetry analysis of protein-protein interactions

Tris-Cl pH 8.0 - MgCl2

Buffer 2 - Calorimetry

Calorimetry analysis of protein-protein interactions

Tris-Cl pH 8.0 - MgCl2

Pages