General nanoCLAMP(Cys) product information, v 1.0
|Quantity:||10 nmol (+/-0.5 nmol)|
|Form Supplied:||Freeze-dried, 90% pure nanoCLAMP protein. Protein was freeze-dried in 100 ul (+/- 5%, depending upon measured freeze-drying concentration)10 mM MOPS, pH 6.5|
|Conjugation:||Protein contains a lone, C-terminal Cysteine|
|Stability / Storage:||Store ≤ 4C until resuspension. After resuspension, aliquot and freeze.|
Resuspension instructions prior to maleimide or haloacetyl conjugation
- Collect product at bottom of tube by centrifugation for 10 sec.
- Add appropriate volume of NRB1 (for maleimide conjugation) or NRB2 (for haloacetyl conjugation), vortex gently for 10 x 5 sec, and then pipette up and down without foaming for 2 minutes.
- Add freshly made TCEP (pH 7 in H2O) to 2 mM and incubate 30 minutes to reduce Cys, then conjugate immediately. There is no need to remove TCEP prior to conjugation.
- Store protein at 4C (1 week) or aliquot and freeze for long term storage. Note: buffer exchange protein into same buffer with fresh 2 mM TCEP using Zeba™ Micro Spin Desalting Columns, 7K MWCO, 75 μL or 0.5 ml (Thermo), prior to conjugation if you suspect TCEP has been oxidized.
NRB1: 50 mM Tris, 5 mM EDTA, 0.05% Tween20, 0.5M Arg, pH 7.2 (1 ml)
NRB2: 50 mM Tris, 5 mM EDTA, 0.05% Tween20, 0.5M Arg, pH 8.5 (1 ml)
Reagents not supplied
This product (“Product”) is warranted to perform in conformance with this Product specification and to be free from defects in material or workmanship for up to one year from time of sale. This product is for research use only. Nectagen is under no obligation to replace products as the result of accidents, misuse, disaster, force majeure, fault or negligence of purchaser, use of product in a manner unintended for its use, or improper storage and handling. No claim of suitability for FDA regulated applications is made.