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Outside Materials
| Title | Description | Vendor |
Catalog Number |
|---|---|---|---|
| TG1 Electroporation-Competent Cells | For use in phage display library preparation, ≥1E10 transformations/µg | Agilent Technologies | 100123 |
| TEV Protease (TurboTEV) | GST- and His-tagged | Eton Bioscience | 1500020012 |
| Pierce Maleimide Activated Plates (8 well strips) | ThermoFisher Scientific | 15150 | |
| Pierce Maleimide Activated Plates (white, 96-well), | ThermoFisher Scientific | 15152 | |
| Pierce Maleimide Activated Plates (black, 96-well) | ThermoFisher Scientific | 15153 | |
| UltraLink™ Iodoacetyl Resin | ThermoFisher Scientific | 20401 | |
| SulfoLink™ Coupling Resin | Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules. | ThermoFisher Scientific | 20401 |
| PreScission Protease | GST-tagged HRV 3C protease | GE Healthcare Life Sciences | 27-0843-01 |
| Ni-NTA Agarose - Max Pressure 2.8 psi | For purification of His-tagged proteins by gravity-flow chromatography | Qiagen | 30230 |
| Oak Ridge High Speed Polycarbonate Centrifuge Tube - 30 ml | 30 ml polypropylene copolymer, with polypropylene screw cap) | ThermoFisher Scientific | 3139 |
| 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo) | ThermoFisher Scientific | 34028 | |
| Ultra Yield Flask - 2.5 l | Fisher Scientific | 50-996-056 | |
| PureCube Maleimide Activated Agarose | Cube Biotech | 51103 | |
| TG1 Electrocompetent Cells | Lucigen | 60502 | |
| Dynabeads® MyOne™ Streptavidin T1 | ThermoFisher Scientific | 65601 | |
| Anti-E. coli RNA Polymerase ImmunoAffinity Resin (Softag 1) | mAb clone NT73 | BioLegend | 673601 |
| Anti-RNA Polymerase TFIIB ImmunoAffinity Resin (Softag 3) | mAb clone IIB8 | BioLegend | 673701 |
| HRV 3C Protease | 6xHN tagged | Takara-Clontech | 7360 |
| B-PER | ThermoFisher Scientific | 78248 | |
| HRV3C Protease protein (His tag) | Fitzgerald Industries International | 80R-2557 | |
| Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL | Spin column for microfuges. 7 kD MW cutoff. | ThermoFisher Scientific | 89882 |
| anti-His-HRP | Sigma-Aldrich | A7058 | |
| Agarose beads, Iodoacetyl functional | Nanocs | AR-IA-1 | |
| Agarose beads, maleimide functional | Nanocs | AR-ML-1 | |
| BL21 (DE3) Competent E. coli | New England Biolabs | C2527I | |
| NiCo21(DE3) Competent E. coli | New England Biolabs | C2529H | |
| LOBSTR E. coli Expression Strain | Kerafast | EC1001 | |
| BcMag™ Iodoacetyl-activated Magnetic Beads, 1 um | Bioclone | FG-101 | |
| BcMag™ Iodoacetyl-activated Magnetic Beads, 5 um | Bioclone | FG-103 | |
| BcMag™ Long-Arm-Iodoacetyl-activated Magnetic Beads, 1 um | Bioclone | FG-105 | |
| BcMag™ Long-Arm-Iodoacetyl-activated Magnetic Beads, 5 um | Bioclone | FG-107 | |
| Sulfhydryl-Reactive Resin | ProteoChem | g4101-15ml | |
| High-Affinity Iodoacetyl Resin | GenScript | L00403 | |
| HRV 3C Protease (Human Rhinovirus 3C Protease, PreScission Site) | His-tagged | Sino Biological Inc. | S3CP01-2000 |
| TMB for membranes | Sigma-Aldrich | T0565-100ML | |
| Mix & Go Competent Cells - Strain TG1 | Zymo Research | T3017 | |
| TEV Protease | His- and GST-tagged | Sigma-Aldrich | T4455-1MG |
| PreScission Protease (PSP), His, Human | N-terminal His-tagged HRV 3C protease | GenScript | Z03092-500 |