Outside Materials

Title Description Vendor Catalog Number
Zeba™ Spin Desalting Columns, 7K MWCO, 0.5 mL Spin column for microfuges. 7 kD MW cutoff. ThermoFisher Scientific 89882
Ni-NTA Agarose - Max Pressure 2.8 psi For purification of His-tagged proteins by gravity-flow chromatography Qiagen 30230
Oak Ridge High Speed Polycarbonate Centrifuge Tube - 30 ml 30 ml polypropylene copolymer, with polypropylene screw cap) ThermoFisher Scientific 3139
Ultra Yield Flask - 2.5 l Fisher Scientific 50-996-056
Mix & Go Competent Cells - Strain TG1 Zymo Research T3017
TG1 Electroporation-Competent Cells For use in phage display library preparation, ≥1E10 transformations/µg Agilent Technologies 100123
BcMag™ Long-Arm-Iodoacetyl-activated Magnetic Beads, 5 um Bioclone FG-107
BcMag™ Long-Arm-Iodoacetyl-activated Magnetic Beads, 1 um Bioclone FG-105
BcMag™ Iodoacetyl-activated Magnetic Beads, 5 um Bioclone FG-103
BcMag™ Iodoacetyl-activated Magnetic Beads, 1 um Bioclone FG-101
Pierce Maleimide Activated Plates (black, 96-well) ThermoFisher Scientific 15153
Pierce Maleimide Activated Plates (white, 96-well), ThermoFisher Scientific 15152
PureCube Maleimide Activated Agarose Cube Biotech 51103
Agarose beads, maleimide functional Nanocs AR-ML-1
Agarose beads, Iodoacetyl functional Nanocs AR-IA-1
Sulfhydryl-Reactive Resin ProteoChem g4101-15ml
High-Affinity Iodoacetyl Resin GenScript L00403
UltraLink™ Iodoacetyl Resin ThermoFisher Scientific 20401
HRV 3C Protease (Human Rhinovirus 3C Protease, PreScission Site) His-tagged Sino Biological Inc. S3CP01-2000
PreScission Protease GST-tagged HRV 3C protease GE Healthcare Life Sciences 27-0843-01
PreScission Protease (PSP), His, Human N-terminal His-tagged HRV 3C protease GenScript Z03092-500
HRV3C Protease protein (His tag) Fitzgerald Industries International 80R-2557
HRV 3C Protease 6xHN tagged Takara-Clontech 7360
TEV Protease (TurboTEV) GST- and His-tagged Eton Bioscience 1500020012
TEV Protease His- and GST-tagged Sigma-Aldrich T4455-1MG
LOBSTR E. coli Expression Strain Kerafast EC1001
NiCo21(DE3) Competent E. coli New England Biolabs C2529H
Anti-RNA Polymerase TFIIB ImmunoAffinity Resin (Softag 3) mAb clone IIB8 BioLegend 673701
Anti-E. coli RNA Polymerase ImmunoAffinity Resin (Softag 1) mAb clone NT73 BioLegend 673601
SulfoLink™ Coupling Resin Thermo Scientific SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups for covalent immobilization of cysteine-peptides and other sulfhydryl molecules. ThermoFisher Scientific 20401
Dynabeads® MyOne™ Streptavidin T1 ThermoFisher Scientific 65601
TG1 Electrocompetent Cells Lucigen 60502
BL21 (DE3) Competent E. coli New England Biolabs C2527I
B-PER ThermoFisher Scientific 78248
Pierce Maleimide Activated Plates (8 well strips) ThermoFisher Scientific 15150
1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo) ThermoFisher Scientific 34028
TMB for membranes Sigma-Aldrich T0565-100ML
anti-His-HRP Sigma-Aldrich A7058