DAPI

Synonym(s)
2-[4-(aminoiminomethyl)phenyl]-1H-Indole-6-carboximidamide; DAPI
Wikipedia Entry
DAPI
Optical Properties
Solvent Dimethyl Sulfoxide, 100% (vol:vol) Water, 100% (vol:vol)
Max Absorption 353 nm 344 nm
Max Emission 465 nm 487 nm
Extinction Coefficient 27,000 cm-1M-1 27,000 cm-1M-1
Quantum Yield 0.58 0.04
Stokes Shift 112 nm 143 nm
Brightness Index 16 1
Outside Data
Repository Accession Number Note
Fluorophores.org 1013 Bound to DNA
Fluorophores.org 274 10 mM Tris pH 7.4
Fluorophores.org 276 Bound to ssDNA in 10 mM Tris pH 7.4
Fluorophores.org 275 Bound to dsDNA in 10 mM Tris pH 7.4
Fluorophores.org 1053 DMSO
Fluorophores.org 1054 Water
Reference(s)
Reference Title PMID/ISBN
McNamara et al. 2017 Microscopy and Image Analysis 28696557
Ingredient List
1 DAPI Dihydrochloride, 5 mg/ml
2 DAPI Dilactate, 10 mM