Strains

Strain Name Description
E. coli NEc1 E. coli strain derived from BL21(DE3) and used for minimizing SlyD contamination in 6-His tagged protein preparations. Reduced contamination of recombinant His-tagged protein or polyhistidine-tagged protein purified by immobilized metal affinity chromatography (IMAC)
E. coli XL1-Blue E. coli strain used for routine cloning with blue/white selection
E. coli BL21(DE3) E. coli strain used for high level expression of proteins from T7 promoter
E. coli TG1 E. coli strain used for phage display