nanoCLAMP trxA-A1

nanoCLAMP trxA-A1 is a single domain antibody mimetic recognizing thioredoxin, thioredoxin fusions and thioredoxin-tagged proteins. nanoCLAMP trxA-A1 anti-thioredoxin beads are useful for thioredoxin purification, thioredoxin chromatography, thioredoxin immunoprecipitation, thioredoxin isolation, thioredoxin removal, and thioredoxin depletion. Nectagen's thioredoxin beads can be used to purify thioredoxin-tagged proteins or remove thioredoxin tags cleaved from purified protein. nanoCLAMP trxA-A1 is an alternative to thioredoxin antibodies.

Products

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-trxA-A1(Resin)	Affinity chromatography resin for E. coli Thioredoxin 1. nanoCLAMP trxA-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of ca. 2 mg (46 nmol) Thioredoxin-fusion protein per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for thioredoxin purification, thioredoxin immunoprecipitation (IP), thioredoxin chromatography, thioredoxin isolation, thioredoxin pulldown, thioredoxin removal, and thioredoxin depletion. Recognizes thioredoxin, thioredoxin-fusions and thioredoxin-tagged proteins. Alternative to thioredoxin antibody beads.	$475	$975	Quantity

Applications

Figure	Application	Outcome
	Thioredoxin purification by affinity chromatography with nanoCLAMP trxA-A1(Resin), batch mode from E. coli lysate	Successful

Targets
Consensus Target	Thioredoxin-1 (E. coli)
Confirmed Targets	Thioredoxin-1 (E. coli)
Non-Reactive Targets

Biophysical Data

Extinction Coefficients

Variant	ε (280 nm)	Solvent	Determination Method
nanoCLAMP trxA-A1(Cys)	31,720 M^-1cm^-1	6 M Guanidine HCl	Pace et al. 1995
nanoCLAMP trxA-A1(Cys)	32,430 M^-1cm^-1	Water	Pace et al. 1995

Variants

nanoCLAMP trxA-A1(Cys)

nanoCLAMP trxA-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys

Conjugates

nanoCLAMP trxA-A1(Resin)

trxA-A1(Cys) coupled to Sulfolink™ resin