nanoCLAMP SMT3-A1

nanoCLAMP SMT3-A1 is a single domain antibody mimetic recognizing yeast SUMO, human SUMO2, human SUMO3, SUMOstar and SUMO-tagged proteins. Validated applications include affinity chromatography and sandwich ELISA. nanoCLAMP SMT3-A1 anti-SUMO beads are useful for SUMO purification, SUMO immunoprecipitation (IP), SUMO pulldown, SUMO chromatography and SUMO isolation. nanoCLAMP SMT3-A1 represents an alternative to SUMO antibodies and SUMO nanobodies. SMT3-A1 beads can be used for cleaved SUMO-tag removal from purified proteins.

Products

Catalog No.	Description	Academic Price	Commercial Price (including license fee)	Quantity
nC-SMT3-A1(Cys)	nanoCLAMP SMT3-A1 recognizing yeast SUMO and human SUMO-2 and SUMO-3. Purified, lyophilized protein with unique C-terminal cysteine ready for conjugation. 10 nmol (180 ug).	$275	$525	Quantity
nC-SMT3-A1(Resin)	Affinity chromatography resin for yeast SUMO and human SUMO-2 and SUMO-3. nanoCLAMP SMT3-A1 coupled to 6% agarose beads via thioether bond to C-terminal cysteine. Binding capacity of > 4.1 mg (175 nmol) SUMO-thioredoxin fusion per ml resin. 0.75 ml resin in 1.5 ml suspension. Useful for SUMO purification, SUMO immunoprecipitation (IP), SUMO chromatography, SUMO isolation, SUMO pulldown, SUMO removal, and SUMO depletion. Recognizes SUMO, SUMO-fusions and SUMO-tagged proteins. Alternative to SUMO antibody beads and SUMO nanobody beads.	$475	$975	Quantity
pET(nC-SMT3-A1)	T7 expression plasmid for nanoCLAMP SMT3-A1. 200 ng lyophilized plasmid DNA	$65	$2000	Quantity

Applications

Figure	Application	Outcome
	SUMO purification by affinity chromatography with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate	Successful
	Western blot analysis of recombinant SMT3 (yeast SUMO) using SMT3-A1(Cys) as primary and anti-His-HRP as secondary	Successful
	SMT3 (yeast SUMO) sandwich ELISA with immobilized SMT3-A1(Cys)	Successful
	Determination of dissociation constant of SMT3-A1(Cys) for SMT3 (yeast SUMO) by microscale thermophoresis	Successful
	SUMOstar affinity purification with nanoCLAMP SMT3-A1(Resin), batch mode from E. coli lysate	Successful

Targets
Consensus Target	SUMO (yeast)
Confirmed Targets	SUMO (yeast), SUMO2 (human), SUMO3 (human), SUMOstar
Non-Reactive Targets

Biophysical Data

Binding Data

Type	Value	Target	App
Kd	163 nM	SUMO (yeast)	Determination of dissociation constant of SMT3-A1(Cys) for SMT3 (yeast SUMO) by microscale thermophoresis

Extinction Coefficients

Variant	ε (280 nm)	Solvent	Determination Method
nanoCLAMP SMT3-A1(Cys)	29,160 M^-1cm^-1	6 M Guanidine HCl	Pace et al. 1995
nanoCLAMP SMT3-A1(Cys)	29,450 M^-1cm^-1	Water	Pace et al. 1995

Variants

nanoCLAMP SMT3-A1(Cys)

nanoCLAMP SMT3-A1 with N-terminal His-tag and C-terminal GS13 linker and unique Cys

Conjugates

nanoCLAMP SMT3-A1(Resin)

SMT3-A1(Cys) coupled to Sulfolink™ resin

Reference(s)

Reference	Title	PMID/ISBN
Suderman et al. 2017	Development of Polyol-Responsive Antibody Mimetics for Single-Step Protein Purification	28428153